Pregnancy and Disease Testing

131112_Mitchw_0167_450pxDairy One’s PregCheck laboratory was established in March 2013.  Using ELISA technology, the laboratory can determine the pregnancy status of bovine with 98% or greater accuracy.  The ability to test blood as well as milk gives customers greater  flexibility when integrating these tests into their herd management procedures.

The DG29™ blood pregnancy test was developed by Conception Animal Reproduction Technologies, and allows a cow to be tested at only 29 days post breeding and 90 days post calving.  This test can also be used for pregnancy detection in goats and sheep. Blood samples from eligible cows may be submitted using either full test kits or tube only kits supplied by Dairy One.

The milk pregnancy test was developed by IDEXX Laboratories, one of the world’s leading diagnostic testing manufacturers.  The use of milk as a medium for pregnancy testing has allowed customers to receive more data from the samples they are already submitting for component testing.  While most of the milk pregnancy samples that the laboratory receives are sampled during regular component testing intervals, samples can be submitted at any time by ordering a milk pregnancy test kit from Dairy One’s supply department.  Cows can be tested as early as 28 days post breeding and 60 days post calving.

Milk Pregnancy samples – all are tested at the Dairy One Ithaca Lab

Two options exist for milk pregnancy testing:

Option 1—Test Day Samples

Individual DHIA cow samples taken on test day can be analyzed at a cost of $4.50/sample.

Option 2—Between Test Days *Now with Free Shipping*

Strip samples from individual cows can be submitted between test days and analyzed at a cost of $4.50/sample plus shipping. This test can also be used for pregnancy detection in goats.

Dairy One provides free shipping when farms submit 10 or more samples for analysis at one time using the special Dairy One test kits and sending the samples either on the Dairy One Sample Transportation System or using the preprinted UPS labels in the Dairy One test kit.

For your convenience, you can order a Dairy One test kit with 50 pre-preserved vials, shipping envelopes and pre-printed UPS labels for $10.00. This fee helps cover the cost of the materials. To order a sample kit, please email supply@dairyone.com or call the office at 800-344-2697. Chick here to download a PDF version of the  Sample Submission Form.

BOTH OPTIONS *Now Offering free “RECHECK”s*

While most milk pregnancy test results provide a “YES” or “NO” answer, a small number of milk pregnancy samples cannot provide a definite answer on whether the cow is pregnant. Instead the cow is listed as “RECHECK.” Dairy One does not charge for any test that has a “RECHECK” result as long as the sample was taken in accordance with the manufacturer’s label. Eligible cows currently must be at least 60 days in milk after calving and 28 days post breeding.

For information on IDEXX Milk Pregnancy Test performance and sensitivity, please refer to the Validation Data Report.

         FAQ

  1. How do I start testing?
    If your herd is already being tested for components through Dairy One, just let your technician know that you would like to begin pregnancy testing.  If you do not use Dairy One’s other milk testing services, call us at 800-344-2697, ext 7 to set up an account and get sampling supplies.
  2. How much does the test cost?
    The test is $4.50 per sample.  Any sample that yields a “Recheck” result will not be invoiced.  A sampling kit of 50 vials, customized paperwork, and free sample shipping labels is available for purchase for $10.00 including the cost of shipping the kit to you.  Be sure to send 10 or more samples in each shipment to take advantage of the free shipping labels included in the kit.
  3. I’m sending milk samples for pregnancy testing; do they need to be refrigerated?
    No, but please keep them refrigerated as long as possible before shipping.  While the test can still be performed on spoiled samples, it’s more difficult to test a spoiled sample.
  4. When does the animal health lab test milk pregnancy samples?
    The lab tests milk pregnancy samples Monday-Friday with the exception of major holidays.  The lab also tests milk pregnancy samples either Saturday or Sunday on most weekends.
  5. Can I use the test for both cows and goats?
    Yes! Each animal must be at least 28 days post breeding and 60 days post calving.
  6. How does the test work?
    The IDEXX milk pregnancy test detects the presence of Pregnancy Associated Glycoproteins (PAGs).  These are not hormones that naturally fluctuate, but rather substances that are present in an animal only when it is pregnant.
  7. How accurate is the test?
    The IDEXX bovine milk pregnancy test is 98% accurate, with false-negative results being quite rare.
  8. What do these results mean?
    A “Positive” result indicates that the animal is pregnant.  An “Open” result indicates that the animal is not pregnant.  A “Recheck” result indicates that the animal might be experiencing a loss of pregnancy.
  9. When can I expect to receive results?
    Once your samples arrive in the Animal Health Diagnostics lab, you can expect results within 24 hours.  If your herd is also being tested for components in our milk lab, your pregnancy samples usually arrive in the animal health lab either the same day or the next day.
  10. How are results sent to me?
    All results are sent via the US Postal Service.  We also send results via email if one is provided.  All results are also downloaded to Dairy Comp automatically.
  11. I have not received results yet, what happened?
    You can reach the animal health lab during regular business hours at 1-800-344-2697 ext 6.  You can also reach us at ahd@dairyone.com.  Please make sure we have the most current email address on file for you.
  12. My herd’s samples have already been collected and sent to the milk lab, but I’d like some to be tested for pregnancy.  How do I let you know?
    Please call us as soon as possible at 1-800-344-2697 ext 4.
  13. When am I going to be billed?
    Billing for milk pregnancy tests is done several times throughout the month. You will not be billed if you have paid in advance.

Blood Pregnancy samples

We are using proven technology by Conception Animal Reproduction Technologies. For best results, the test should be conducted on cows or heifers 29 days or more post breeding. Cows must also be at least 90 days post calving before blood samples are collected. We sell test kits that include the price of the lab analysis as shown below. Chick here to download a PDF version of the  Sample Submission Form.

CodeDescriptionUnitsPricePer SampleShippingTotal
DGK6DG29 Complete 6 Test Kit6$25.00$4.167N/A$25.00
DGK24DG29 Complete 24 Test Kit24$85.00$3.542N/A$85.00
DGK48DG29 Complete 48 Test Kit48$165.00$3.436N/A$165.00
DGK72DG29 Complete 72 Test Kit72$243.00$3.375N/A$243.00
DGK144DG29 Complete 144 Test Kit144$475.00$3.299N/A$475.00
Full kits include: test analysis, red-top blood tubes, disposable needles, needle holder, hazardous material shipping containers, shipping to the farm
DGT48DG29 48 Testing Samples48$148.00$3.083$8.50$156.50
DGT72DG29 72 Testing Samples72$201.00$2.792$8.50$209.50
DGT144DG29 144 Testing Samples144$381.00$2.646$10.00$391.00
DGT288DG29 288 Testing Samples288$720.00$2.50$12.00$732.00
Tube kits include: test analysis, red-top blood tubes, hazardous material shipping containers (no needles and no shipping to farm)

         FAQ

  1. How do I start testing?
    Contact us to set up an account and get sampling supplies.  Our supply department can help get you started, contact them at 1-800-344-2697 ext 2233.
  2. How much does the test cost?
    The cost of the test is included in the cost of sampling supplies.  If you choose to use your own sampling supplies, the test is $4.17 per sample.  If you would like to utilize Dairy One’s sample transportation network, the cost is $7 for each time it is used (regardless of the number of samples).
  3. I’m sending blood samples for pregnancy testing; do they need to be refrigerated?
    No, but please note that cross contamination (ie using one needle for more than one animal) can cause samples to coagulate. Please take care to avoid this as it makes handling the sample difficult in the lab.
  4. When does the animal health lab test blood pregnancy samples?
    The animal health lab tests blood pregnancy samples Monday through Friday with the exception of major holidays.
  5. What animals can I use this test for?
    This test can be used on cows, goats, and sheep.  Each animal must be at least 29 days post breeding and 90 days post calving.
  6. How does the test work?
    The DG29 blood pregnancy test detects the presence of Pregnancy Associated Glycoproteins (or PAGs, for short).  These are not hormones that naturally fluctuate, but rather substances that are present in an animal only when it is pregnant.
  7. How accurate is the test?
    The DG29 blood pregnancy test is 99% accurate.
  8. What do these results mean?
    A “Positive” result indicates that the animal is pregnant.  An “Negative” result indicates that the animal is not pregnant.  Please pay close attention to any letters displayed in the “comments” section of the report.  A “Call Us” result usually indicates that the days post breeding and/or days post calving requirements were not met.
  9. When can I expect to receive results?
    Once your samples arrive in the animal health lab, you can expect results within 24 hours.
  10. How are results sent to me?
    Results will be sent to you as indicated on the sample submission form.  If an email or fax number are not provided we will send the results via US Postal Service. All results for existing Dairy One customers are also uploaded to Dairy Comp.
  11. I have not received results yet, what happened?
    You can reach the animal health lab during regular business hours at 1-800-344-2697 ext 2174.  You can also reach us at ahd@dairyone.com.  Your samples may have not arrived through the mail yet.  Consider using Dairy One transportation network for faster sample results.
  12. How am I going to be billed?
    Billing for blood pregnancy tests is done at the end of the month or you may pay for your sample kit when ordering it though our supply department.

For more information, please e-mail us at PregCheck@DairyOne.com

Johne’s/BVD/Leukosis- These tests require a special report from your DHIA Field Technician to come in with the samples. It must accompany the lab report and be included in the sample box.

Johne’s

Johne’s disease testing is now available directly through Dairy One’s ELISA Laboratory. Performing this testing at Dairy One improves both flexibility and timing for our customers. Results are typically available within 24 to 48 hours from the time samples arrive at the lab and a veterinarian is not longer required to be involved. Johne’s testing can now be more easily incorporated into an overall herd health testing routine. Individual DHIA cow samples with preservatives can be analyzed at a cost of $6/sample.

         FAQ

  1. How do I start testing?
    If your herd is already being tested for components through Dairy One, just let your technician know that you’d like to begin Johne’s testing.  If you do not use Dairy One’s other milk testing services, contact us to set up an account and get sampling supplies.  Our supply department can help get you started, contact them at 1-800-344-2697 ext 2233
  2. How much does the test cost?
    The test is $6 per sample.  A sampling kit of 50 vials and customized paperwork is available for purchase for $10 including the cost of shipping the kit to you.
  3. I’m sending milk samples for Johne’s testing; do they need to be refrigerated?
    No, but please keep them refrigerated as long as possible before shipping.
  4. When does the animal health lab test Johne’s samples?
    The animal health lab usually tests Johne’s samples a minimum of twice a week.  We may test them more frequently depending on sample volume.
  5. What animals can I use this test for?
    This test can be used only for cows using either milk or blood.
  6. When is the best time to test for the disease?
    The best time to test for Johne’s is when the cow is experiencing the most stress.  This is usually associated with the pre-calving dry-off period.
  7. How does the test work?
    The IDEXX Johne’s Disease test detects the presence of anitbodies associated with the disease;  it does not test for the disease directly. Antibodies are produced by the cow’s body as an immune response to the presence of the disease.
  8. What do these results mean?
    A “Positive” result indicates that the animal has produced antibodies associated with Johne’s Disease.  A “Negative” result indicates that the animal has not produced these antibodies.  A “Suspect” result indicates that the animal may have the disease or may have been exposed to the disease but not contracted it.  Due to the nature of an antibody test, it is recommended that an animal which has tested positive once be retested to ensure that the antibodies that are present are from contraction of the disease as opposed to exposure to the disease that did not result in contraction of the disease.
  9. When can I expect to receive results?
    Depending on sample volume, your results may arrive 1-3 business days after arriving in the animal health lab.  If you need your results sooner, please let us know.  We may be able to accommodate your request.
  10. How are results sent to me?  Are they sent to my veterinarian too?
    All results are sent via the US Postal Service.  We also send results via email if one is provided.  All results are also uploaded to Dairy Comp automatically.  We do not send results to veterinarians.
  11. I have not received results yet, what happened?
    You can reach the animal health lab during regular business hours at 1-800-344-2697 ext 2174.  You can also reach us at ahd@dairyone.com.  We may have an invalid mailing or email address on file for you.
  12. My herd’s samples have already been collected and sent to the lab, but I’d like some to be tested for Johne’s.  How do I let you know?
    Please call us as soon as possible at 1-800-344-2697 ext 2160 or 2151.  If no one can be reached at those extensions, use 2140.
  13. When am I going to be billed?
    Billing for Johne’s tests is done at increments throughout the month; it is usually done once a week.  You will not be billed if you have paid in advance.

BVD

BVD Tank samples through Cornell University’s Animal Health Diagnostic Center

  • Samples submitted to this laboratory must be submitted by a licensed Veterinarian
  • This analysis is performed for bulk tank samples only at a cost of  $60 per sample. A composite sample for multiple cows is recommended unless there is known BVD in the herd.
  • Sample submission: submit within 24 hours.
  • Use a well cleaned soda bottle or jar with screw on lid
  • Size of Sample: 200-700 ml depending on # cows in sample (400 max cows)
  • Sample: Not Frozen, not spoiled or curdled
  • Transportation: keep sample cold (NOT frozen).
  • Turnaround: 3-5 days

BVD Samples through Antel Bio

  • A bulk tank sample can be used with a maximum of 400 cows contributing to the bulk tank. The cost is $45 per bulk tank sample plus shipping.
  • Individual DHIA cow samples, with preservatives can be also be analyzed at a cost of $6/sample plus shipping.

         FAQ

  1. How do I start testing?
    Contact us to set up an account and get sampling supplies.  Our supply department can help get you started, contact them at 1-800-344-2697 ext 2233.
  2. How much does the test cost?
    The test is $5.50 per sample.  A sampling kit of 50 vials and customized paperwork is available for purchase for $10 including the cost of shipping the kit to you.
  3. When does the animal health lab test BVD samples?
    The animal health lab usually tests BVD samples according to sample volume.  We may test them more frequently depending on sample volume.
  4. What animals can I use this test for?
    This test can be used only for cows meeting the following specifications:
    – Blood can be submitted for testing from calves that are either precolostral or more than 3 months old can be tested.
    – Ear notches or milk can be used to test any cow.
  5. When is the best time to test for the disease?
    The best time to test for BVD is immediately after a calf born.
  6. How does the test work?
    The IDEXX BVD test detects the presence of anitbodies associated with the disease;  it does not test for the disease directly. Antibodies are produced by the cow’s body as an immune response to the presence of the disease.
  7. What do these results mean?
    A “Positive” result indicates that the animal has produced antibodies associated with BVD.  A “Negative” result indicates that the animal has not produced these antibodies.  A “Suspect” result indicates that the animal may have the disease or may have been exposed to the disease but not contracted it.  Due to the nature of an antibody test, it is recommended that an animal which has tested positive once be retested to ensure that the antibodies that are present are from contraction of the disease as opposed to exposure to the disease that did not result in contraction of the disease.  A retest sample is recommended to be taken at least 2 weeks after the initial positive sample.
  8. When can I expect to receive results?
    Depending on sample volume, your results may arrive 1-3 business days after arriving in the animal health lab.  If you need your results sooner, please let us know.  We may be able to accommodate your request.
  9.  How are results sent to me Are they sent to my veterinarian too?
    All results are sent via the US Postal Service.  We also send results via email if one is provided.  All results are also uploaded to Dairy Comp automatically.  We do not send results to veterinarians unless the vet’s email is included on the paperwork.
  10. I have not received results yet, what happened?
    You can reach the animal health lab during regular business hours at 1-800-344-2697 ext 2174.  You can also reach us at ahd@dairyone.com.  We may have an invalid mailing or email address on file for you.
  11. My herd’s samples have already been collected and sent to the lab, but I’d like some to be tested for BVD.  How do I let you know?
    Please call us as soon as possible at 1-800-344-2697 ext 2160 or 2151.  If no one can be reached at those extensions, use 2140.
  12. When am I going to be billed?
    Billing for BVD tests is done at increments throughout the month; it is usually done once a week. You will not be billed if you have paid in advance.

Leukosis

Leukosis samples – all are sent to Antel Bio

This analysis is performed by AntelBio. Individual DHIA cow samples, with preservatives can be analyzed at a cost of $6/sample plus shipping.